Development and RNA-synthesizing activity of coronavirus replication structures in the absence of protein synthesis.
Identifieur interne : 002130 ( Main/Exploration ); précédent : 002129; suivant : 002131Development and RNA-synthesizing activity of coronavirus replication structures in the absence of protein synthesis.
Auteurs : Sjoerd H E. Van Den Worm [Pays-Bas] ; Kèvin Knoops ; Jessika C. Zevenhoven-Dobbe ; Corrine Beugeling ; Yvonne Van Der Meer ; A Mieke Mommaas ; Eric J. SnijderSource :
- Journal of virology [ 1098-5514 ] ; 2011.
Descripteurs français
- KwdFr :
- ARN viral (biosynthèse), Animaux, Biosynthèse des protéines (), Cellules Vero, Cycloheximide (métabolisme), Inhibiteurs de la synthèse protéique (métabolisme), Microscopie de fluorescence, Microscopie électronique à transmission, Protéines virales (métabolisme), Puromycine (métabolisme), Réplication virale, Transcription génétique, Virus de l'hépatite murine (physiologie), Virus du SRAS (physiologie).
- MESH :
- biosynthèse : ARN viral.
- métabolisme : Cycloheximide, Inhibiteurs de la synthèse protéique, Protéines virales, Puromycine.
- physiologie : Virus de l'hépatite murine, Virus du SRAS.
- Animaux, Biosynthèse des protéines, Cellules Vero, Microscopie de fluorescence, Microscopie électronique à transmission, Réplication virale, Transcription génétique.
English descriptors
- KwdEn :
- Animals, Chlorocebus aethiops, Cycloheximide (metabolism), Microscopy, Electron, Transmission, Microscopy, Fluorescence, Murine hepatitis virus (physiology), Protein Biosynthesis (drug effects), Protein Synthesis Inhibitors (metabolism), Puromycin (metabolism), RNA, Viral (biosynthesis), SARS Virus (physiology), Transcription, Genetic, Vero Cells, Viral Proteins (metabolism), Virus Replication.
- MESH :
- chemical , biosynthesis : RNA, Viral.
- chemical , metabolism : Cycloheximide, Protein Synthesis Inhibitors, Puromycin, Viral Proteins.
- drug effects : Protein Biosynthesis.
- physiology : Murine hepatitis virus, SARS Virus.
- Animals, Chlorocebus aethiops, Microscopy, Electron, Transmission, Microscopy, Fluorescence, Transcription, Genetic, Vero Cells, Virus Replication.
Abstract
The RNA replication and transcription complex of coronaviruses is associated with an elaborate reticulovesicular network (RVN) of modified endoplasmic reticulum. Using cycloheximide and puromycin, we have studied the effect of translation inhibition on the RNA synthesis of severe acute respiratory syndrome coronavirus and mouse hepatitis virus. Both inhibitors prevented the usual exponential increase in viral RNA synthesis, with immunofluorescence and electron microscopy indicating that RVN development came to a standstill. Nevertheless, limited RNA synthesis was supported, implying that continued translation is not an absolute requirement and suggesting a direct link between RVN formation and accumulation of coronavirus proteins.
DOI: 10.1128/JVI.00403-11
PubMed: 21430047
Affiliations:
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Le document en format XML
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Zevenhoven-Dobbe</name></author><author><name sortKey="Beugeling, Corrine" sort="Beugeling, Corrine" uniqKey="Beugeling C" first="Corrine" last="Beugeling">Corrine Beugeling</name></author><author><name sortKey="Van Der Meer, Yvonne" sort="Van Der Meer, Yvonne" uniqKey="Van Der Meer Y" first="Yvonne" last="Van Der Meer">Yvonne Van Der Meer</name></author><author><name sortKey="Mommaas, A Mieke" sort="Mommaas, A Mieke" uniqKey="Mommaas A" first="A Mieke" last="Mommaas">A Mieke Mommaas</name></author><author><name sortKey="Snijder, Eric J" sort="Snijder, Eric J" uniqKey="Snijder E" first="Eric J" last="Snijder">Eric J. 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Van Den Worm</name><affiliation wicri:level="1"><nlm:affiliation>Molecular Virology Laboratory, Department of Medical Microbiology, Leiden University Medical Center, 2300 RC Leiden, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Molecular Virology Laboratory, Department of Medical Microbiology, Leiden University Medical Center, 2300 RC Leiden</wicri:regionArea><wicri:noRegion>2300 RC Leiden</wicri:noRegion></affiliation></author><author><name sortKey="Knoops, Kevin" sort="Knoops, Kevin" uniqKey="Knoops K" first="Kèvin" last="Knoops">Kèvin Knoops</name></author><author><name sortKey="Zevenhoven Dobbe, Jessika C" sort="Zevenhoven Dobbe, Jessika C" uniqKey="Zevenhoven Dobbe J" first="Jessika C" last="Zevenhoven-Dobbe">Jessika C. Zevenhoven-Dobbe</name></author><author><name sortKey="Beugeling, Corrine" sort="Beugeling, Corrine" uniqKey="Beugeling C" first="Corrine" last="Beugeling">Corrine Beugeling</name></author><author><name sortKey="Van Der Meer, Yvonne" sort="Van Der Meer, Yvonne" uniqKey="Van Der Meer Y" first="Yvonne" last="Van Der Meer">Yvonne Van Der Meer</name></author><author><name sortKey="Mommaas, A Mieke" sort="Mommaas, A Mieke" uniqKey="Mommaas A" first="A Mieke" last="Mommaas">A Mieke Mommaas</name></author><author><name sortKey="Snijder, Eric J" sort="Snijder, Eric J" uniqKey="Snijder E" first="Eric J" last="Snijder">Eric J. Snijder</name></author></analytic><series><title level="j">Journal of virology</title><idno type="eISSN">1098-5514</idno><imprint><date when="2011" type="published">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Chlorocebus aethiops</term><term>Cycloheximide (metabolism)</term><term>Microscopy, Electron, Transmission</term><term>Microscopy, Fluorescence</term><term>Murine hepatitis virus (physiology)</term><term>Protein Biosynthesis (drug effects)</term><term>Protein Synthesis Inhibitors (metabolism)</term><term>Puromycin (metabolism)</term><term>RNA, Viral (biosynthesis)</term><term>SARS Virus (physiology)</term><term>Transcription, Genetic</term><term>Vero Cells</term><term>Viral Proteins (metabolism)</term><term>Virus Replication</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral (biosynthèse)</term><term>Animaux</term><term>Biosynthèse des protéines ()</term><term>Cellules Vero</term><term>Cycloheximide (métabolisme)</term><term>Inhibiteurs de la synthèse protéique (métabolisme)</term><term>Microscopie de fluorescence</term><term>Microscopie électronique à transmission</term><term>Protéines virales (métabolisme)</term><term>Puromycine (métabolisme)</term><term>Réplication virale</term><term>Transcription génétique</term><term>Virus de l'hépatite murine (physiologie)</term><term>Virus du SRAS (physiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>RNA, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Cycloheximide</term><term>Protein Synthesis Inhibitors</term><term>Puromycin</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>ARN viral</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Protein Biosynthesis</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Cycloheximide</term><term>Inhibiteurs de la synthèse protéique</term><term>Protéines virales</term><term>Puromycine</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Virus de l'hépatite murine</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Murine hepatitis virus</term><term>SARS Virus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Chlorocebus aethiops</term><term>Microscopy, Electron, Transmission</term><term>Microscopy, Fluorescence</term><term>Transcription, Genetic</term><term>Vero Cells</term><term>Virus Replication</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Biosynthèse des protéines</term><term>Cellules Vero</term><term>Microscopie de fluorescence</term><term>Microscopie électronique à transmission</term><term>Réplication virale</term><term>Transcription génétique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The RNA replication and transcription complex of coronaviruses is associated with an elaborate reticulovesicular network (RVN) of modified endoplasmic reticulum. Using cycloheximide and puromycin, we have studied the effect of translation inhibition on the RNA synthesis of severe acute respiratory syndrome coronavirus and mouse hepatitis virus. Both inhibitors prevented the usual exponential increase in viral RNA synthesis, with immunofluorescence and electron microscopy indicating that RVN development came to a standstill. Nevertheless, limited RNA synthesis was supported, implying that continued translation is not an absolute requirement and suggesting a direct link between RVN formation and accumulation of coronavirus proteins.</div></front></TEI><affiliations><list><country><li>Pays-Bas</li></country></list><tree><noCountry><name sortKey="Beugeling, Corrine" sort="Beugeling, Corrine" uniqKey="Beugeling C" first="Corrine" last="Beugeling">Corrine Beugeling</name><name sortKey="Knoops, Kevin" sort="Knoops, Kevin" uniqKey="Knoops K" first="Kèvin" last="Knoops">Kèvin Knoops</name><name sortKey="Mommaas, A Mieke" sort="Mommaas, A Mieke" uniqKey="Mommaas A" first="A Mieke" last="Mommaas">A Mieke Mommaas</name><name sortKey="Snijder, Eric J" sort="Snijder, Eric J" uniqKey="Snijder E" first="Eric J" last="Snijder">Eric J. Snijder</name><name sortKey="Van Der Meer, Yvonne" sort="Van Der Meer, Yvonne" uniqKey="Van Der Meer Y" first="Yvonne" last="Van Der Meer">Yvonne Van Der Meer</name><name sortKey="Zevenhoven Dobbe, Jessika C" sort="Zevenhoven Dobbe, Jessika C" uniqKey="Zevenhoven Dobbe J" first="Jessika C" last="Zevenhoven-Dobbe">Jessika C. Zevenhoven-Dobbe</name></noCountry><country name="Pays-Bas"><noRegion><name sortKey="Van Den Worm, Sjoerd H E" sort="Van Den Worm, Sjoerd H E" uniqKey="Van Den Worm S" first="Sjoerd H E" last="Van Den Worm">Sjoerd H E. Van Den Worm</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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